research results

research results

Research Results

  1. Sekimizu, N., Paudel, A., Hamamoto, H. (2012) Animal welfare and use of silkworm as a model animal. Drug Discov Ther 6, 226-229. Detail.
  2. Fujiyuki, T., Hamamoto, H., Ishii, K., Urai, M., Kataoka, K., Takeda, T., Shibata, S., Sekimizu, K. (2012) Evaluation of innate immune stimulating activity of polysaccharaides using a silkworm (Bombyx mori) muscle contraction assay. Drug Discov Ther 6, 88-93. Detail.
  3. Dhital, S., Hamamoto, H., Urai, M., Ishii, K. & Sekimizu, K. (2011) Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay. Drug Discov Ther 5, 18-25. Detail.
  4. Matsumoto Y, Sumiya E, Sugita T, Sekimizu K. An invertebrate hyperglycemic model for identification of anti-diabetic drugs. PLoS ONE 30;6(3):e18292 (2011) Detail.
  5. Chikara Kaito, Yuki Saito, Gentaro Nagano, Mariko Ikuo, Yosuke Omae, Yuichi Hanada, Xiao Han, Kyoko Kuwahara-Arai, Tomomi Hishinuma, Tadashi Baba, Teruyo Ito, Keiichi Hiramatsu, Kazuhisa Sekimizu. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus Virulence., PLoS pathog. 7(2): e1001267 (2011) Detail.
  6. Fujiyuki T, Imamura K, Hamamoto H, Sekimizu K. Evaluation of therapeutic effects and pharmacokinetics of antibacterial chromogenic agents in silkworm model of Staphilococcus aureus infection., Drug Discov Ther. 4(5):349-354. (2010) Detail.
  7. Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K. Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. J Biol Chem.285(43): 33338-47, (2010) Detail.
  8. Ishii K, Hamamoto H, Kamimura M, Nakamura Y, Noda H, Imamura K, Mita K, Sekimizu K. The insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm Bombyx mori. J Biol Chem.285(37): 28635-42, (2010) Detail.
  9. Ikuo M, Kaito C, Sekimizu K: The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene. Microb Pathog.49(1-2):1-7, (2010) Detail.
  10. Asami Y, Horie R, Hamamoto H, Sekimizu K:Use of silkworms for identification of drug candidates having appropriate pharmacokinetics from plant sources. BMC Pharmacol.11;10:7, (2010) Detail.
  11. Matsuo, M., Kurokawa, K., Lee, B. L. and Sekimizu, K. (2010) Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus. Biol. Pharm. Bull. 33, 198-203. Detail.
  12. Kurokawa, K., Hamamoto, H., Matsuo, M., Nishida, S., Yamane, N., Lee, B. L., Murakami, K., Maki, H. and Sekimizu, K. (2009) Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model. Antimicrob. Agents Chemother., 53, 4025-4027. Detail.
  13. Usui, K., Miyazaki, S., Kaito, C. and Sekimizu, K. (2009) Purification of soil bacteria exotoxin using silkworm toxicity to measure specific activity. Microb. Pathog., 46, 59-62. Detail.
  14. Oku, Y., Kurokawa, K., Matsuo, M., Yamada, S., Lee, B. L. and Sekimizu, K. (2009) Pleiotropic roles of poly-glycerophosphate synthase of lipoteichoic acid in the growth of Staphylococcus aureus cells. J. Bacteriol., 191, 141-151. Detail.
  15. Hamamoto, H., Tonoike, A., Narushima, K., Horie, R. and Sekimizu,K.(2009) Silkworm as a model animal to evaluate drug candidate toxity and metabolism. Comp.Biochem.Physil., C149,334-339. Detail.
  16. Kurokawa, K., Nishida, S., Ishibashi, M., Mizumura, H., Ueno, K., Yutsudo, T., Maki, H., Murakami, K. and Sekimizu, K. (2008) Staphylococcus aureus MurC participates in L-alanine recognition via Histidine 343, a conserved motif in the shallow hydrophobic pocket. J. Biochem. (Tokyo), 143, 417-424. Detail.
  17. Ishii, K., Hamamoto, H., Sasaki, T., Ikegaya, Y., Yamatsugu, K., Kanai, M., Shibasaki, M. and Sekimizu, K. (2008) Pharmacologic action of oseltamivir on the nervous system. Drug Discoveries and Therapeutics, 2, 24-34. Detail.
  18. Ishii, K., Hamamoto, H., Kamimura, M. and Sekimizu, K. (2008) Activation of the silkworm cytokine by bacterial and fungal cell wall components via a reactive oxygen species-triggered mechanism. J. Biol. Chem., 283, 2185-2191. Detail.
  19. Nagata, M., Kaito, C. and Sekimizu, K. (2008) Phosphodiesterase activity of CvfA is required for virulence in Staphylococcus aureus. J. Biol. Chem., 283, 2176-2184. Detail.
  20. Orihara, Y., Hamamoto, H., Kasuga, H., Shimada, T., Kawaguchi, Y. and Sekimizu, K. (2008) A silkworm-baculovirus model for assessing the therapeutic effects of anti-viral compounds: characterization and application to the isolation of anti-virals from traditional medicines. J. Gen. Virol., 89, 188-194. Detail.
  21. Kaito, C. and Sekimizu, K. (2007) A silkworm model of pathogenic bacterial infection. Drug Discoveries and Therapeutics, 1, 89-93. Detail.
  22. Ishii, K., Hamamoto, H. and Sekimizu, K. (2007) A novel method to suppress the dispersal of Japanese cedar pollen by inducing morphologic changes with weak alkaline solutions. Drug Discoveries and Therapeutics, 1, 124-129. Detail.
  23. Ito, T., Saso, K., Arimitsu, N. and Sekimizu, K. (2007) Defective FESTA/EAF2-mediated transcriptional activation in S-II-deficient embryonic stem cells. Biochem. Biophys. Res. Commun., 363, 603-609. Detail.
  24. Ito, T., Doi, K., Matsumoto, N., Kakihara, F., Noiri, E., Hasegawa, S., Tokunaga, K. and Sekimizu, K. (2007) Lack of polymorphisms in the coding region of the highly conserved gene encoding transcription elongation factor S-II (TCEA1). Drug Discoveries and Therapeutics, 1, 9-11. Detail.
  25. Hattori, K., Hirayama, M., Suzuki, H., Hamamoto, H., Sekimizu, K. and Tamura, H. O. (2007) Cloning and expression of a novel sulfotransferase with unique substrate specificity from Bombyx mori. Biosci. Biotechnol. Biochem., 71, 1044-1051. Detail.
  26. Ashibe, B., Hirai, T., Higashi, K., Sekimizu, K., Motojima, K. (2007) Dual subcellular localization in the endoplasmic reticulum and peroxisomes and a vital role in protecting against oxidative stress of fatty aldehyde dehydrogenase are achieved by alternative splicing. J. Biol. Chem., 282, 20763-20773. Detail.
  27. Li, Y., Kurokawa, K., Reutimann, L., Mizumura, H., Matsuo, M. and Sekimizu, K. (2007) dnaB and dnaI temperature-sensitive mutants of Staphylococcus aureus: evidence for involvement of DnaB and DnaI in synchrony regulation of chromosome replication. Microbiology, 153, 3370-3379. Detail.
  28. Ishibashi, M., Kurokawa, K., Nishida, S., Ueno, K., Matsuo, M. and Sekimizu, K. (2007) Isolation of temperature-sensitive mutations in murC of Staphylococcus aureus. FEMS Microbiol. Lett., 274, 204-209. Detail.
  29. Ikeda, R., Saito, F., Matsuo, M., Kurokawa, K., Sekimizu, K., Yamaguchi, M. and Kawamoto, S. (2007) The contribution of the mannan backbone of cryptococcal glucuronoxylomannan and a glycolytic enzyme of Staphylococcus aureus to contact mediated killing of Cryptococcus neoformans. J. Bacteriol., 189, 4815-4826. Detail.
  30. Kurokawa, K., Kaito, C. and Sekimizu, K., Two-component signaling in virulence of S. aureus: silkworm larvae-pathogenic agents infection model for Virulence. Methods Enzymol., 422, 233-244. Detail.
  31. Koyama, H., Ito, T., Nakanishi, T. and Sekimizu, K. (2007) Stimulation RNA polymerase II transcript cleavage activity contributes to maintain transcriptional fidelity in yeast. Genes to Cells, 12, 547-559. Detail.
  32. Matsumoto, Y., Kaito, C., Morishita, D., Kurokawa, K. and Sekimizu, K. (2007) Regulation of exoprotein gene expression by the Staphylococcus aures cvfB gene. Infect. Immun., 75, 1964-1972. Detail.
  33. Nagata, M., Arimitsu, N., Ito, T. and Sekimizu, K. (2007) Antioxidant N-acetyl-l-cysteine inhibits erythropoietin-induced differentiation of erythroid progenitors derived from mouse fetal liver. Cell Biol Int., 31, 252-256. Detail.
  34. Kaito, C. and Sekimizu, K. (2007) Colony Spreading in Staphylococcus aureus. J Bacteriol., 189, 2553-2557. Detail.
  35. Kaito, C., Morishita, D., Matsumoto, Y., Kurokawa, K. and Sekimizu, K. (2007) Novel DNA binding protein SarZ contributes to virulence in Staphylococcus aureus. Mol Microbiol., 62, 1601-1617. Detail.
  36. Hossain, M. S., Hamamoto, H., Matsumoto, Y., Razanajatovo, I. M., Larranaga, J., Kaito, C., Kasuga, H. and Sekimizu K. (2006) Use of silkworm larvae to study pathogenic bacterial toxins. J. Biochem. (Tokyo), 140, 439-444. Detail.
  37. Ito, T., Arimitsu, N., Takeuchi, M., Kawamura, N., Nagata, M., Saso, K., Akimitsu, N., Hamamoto, H., Natori, S., Miyajima, A. and Sekimizu, K. (2006) Transcription elongation factor S-II is required for definitive hematopoiesis. Mol Cell Biol., 26, 3194-3203. Detail.
  38. Nishida, S., Kurokawa, K., Matsuo, M., Sakamoto, K., Ueno, K., Kita, K. and Sekimizu, K., Identification and characterization of amino acid residues essential for the active site of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus. J. Biol. Chem., 281, 1714-1724. Detail.
  39. Murai, M., Kurokawa, K., Ichihashi, N., Matsuo, M. and Sekimizu, K. (2006) Isolation of a temperature-sensitive dnaA mutant of Staphylococcus aureus. FEMS Microbiol Lett., 254, 19-26. Detail.
  40. Hamamoto, H., Larranaga, J., Sekine, M., Furuchi, T., Katane, M., Homma, H., Matsuki, N. and Sekimizu, K. (2006) D-glutamic acid induced muscle contraction in silkworm larva. in D-Amino Acids: A new Frontier in Amino Acid and Protein Resarch. pp. 249-254. Detail.
  41. Rungruang, T., Kaneko, O., Murakami, Y., Tsuboi, T., Hamamoto, H., Akimitsu, N., Sekimizu, K., Kinoshita, T. and Torii, M. (2005) Erythrocyte surface glycosylphosphatidyl inositol anchored receptor for the malaria parasite. Mol Biochem Parasitol., 140, 13-21. Detail.
  42. Aramaki, Y., Ogawa, K., Toh, Y., Ito, T., Akimitsu, N., Hamamoto, H., Sekimizu, K., Matsusue, K., Kono, A., Iguchi, H. and Takiguchi, S. (2005) Direct interaction between metastasis-associated protein 1 and endophilin 3. FEBS Lett., 579, 3731-3736. Detail.
  43. Hamamoto, H., Kamura, K., Razanajatovo, I. M., Murakami, K., Santa, T. and Sekimizu, K. (2005) Effects of molecular mass and hydrophobicity on transport rates through non-specific pathways of the silkworm larva midgut. Int. J. Antimicrob. Agents 26, 38-42. Detail.
  44. Kawamura, N., Kurokawa, K., Ito, T., Hamamoto, H., Koyama, H., Kaito, C. and Sekimizu, K. (2005) Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation. Genes Cells, 10, 477-487. Detail.
  45. Kaito, C., Kurokawa, K., Matsumoto, Y., Terao, Y., Kawabata, S., Hamada, S. and Sekimizu, K. (2005) Silkworm-pathogenic bacteria infection model for identification of novel virulence genes. Mol. Microbiol., 56, 34-44. Detail.
  46. Sekimizu, K., Larranaga, J., Hamamoto, H., Sekine, M., Furuchi, T., Katane, M., Homma, H. and Matsuki, N. (2005) D-Glutamic acid-induced muscle contraction in the silkworm, Bombyx mori. J. Biochem. (Tokyo), 137, 199-203. Detail.
  47. Hamamoto H and Sekimizu K. (2005) Evaluation of the therapeutic effects of antibiotics using silkworm as an animal model. Res. Adv. Antimicrob. Agents Chemother, 5, 1-23.
  48. Shiokawa, K., Takayama, E., Higo, T., Kuroyanagi, S., Kaito, C., Hara, H., Kajitani, M., Sekimizu, K., Tadakuma, T., Miura, K., Igarashi, K. and Yaoita, Y. (2005) Occurrence of pre-MBT synthesis of caspase-8 mRNA and activation of caspase-8 prior to execution of SAMDC (S-adenosylmethionine decarboxylase)-induced, but not p53-induced, apoptosis in Xenopus late blastulae. Biochem. Biophys. Res. Commun., 336, 682-691. Detail.
  49. Yamaguchi, S., Katagiri, S., Sekimizu, K., Natori, S. and Homma, K. J. (2005) Involvement of EDTP, an Egg-Derived Tyrosine Phosphatase, in the Early Development of Drosophila melanogaster. J Biochem. (Tokyo), 138, 721-728. Detail.
  50. Hossain, M. S., Kurokawa, K. and Sekimizu, K. (2004) Induction of fusion-competent myoblast-specific gene expression during myogenic differentiation of Drosophila Schneider cells by DNA double-strand breaks or replication inhibition. Biochim. Biophys. Acta, 1743, 176-186. Detail.
  51. Nakata, A., Ito, T., Nagata, M., Hori, S. and Sekimizu, K. (2004) GRIP1tau, a novel PDZ domain-containing transcriptional activator, cooperates with the testis-specific transcription elongation factor SII-T1. Genes Cells, 9, 1125-1135. Detail.
  52. Hossain, M. S., Kurokawa, K., Akimitsu, N. and Sekimizu, K. (2004) DNA topoisomerase II is required for the G0-to-S phase transition in Drosophila Schneider cells, but not in yeast. Genes Cells, 9, 905-917. Detail.
  53. Ishida, T., Akimitsu, N., Kashioka, T., Hatano, M., Kubota, T., Ogata, Y., Sekimizu, K. and Katayama, T. (2004) DiaA, a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication. J. Biol. Chem., 279, 45546-45555. Detail.
  54. Akimitsu, N., Kim, H. S., Hamamoto, H., Kamura, K., Fukuma, N., Arimitsu, N., Ono, K., Wataya, Y., Torii, M., Sekimizu, K. (2004) Duffy antigen is important for the lethal effect of the lethal strain of Plasmodium yoelii 17XL. Parasitol. Res., 93, 499-503. Detail.
  55. Saito, M., Santa, T., Tsunoda, M., Hamamoto, H. and Usui, N. (2004) An automated analyzer for vancomycin in plasma samples by column-switching high-performance liquid chromatography with UV detection, Biomed. Chromatogr., 18, 735-738. Detail.
  56. Jilma, B., Akimitsu, N., Fukuma, N., Sekimizu K, Jilma-Stohlawetz, P. (2004) Man, mouse and Duffy genotype-phenotype-specific pharmacokinetics of monocyte chemotactic protein-1. Transfus. Med., 14, 251-252. Detail.
  57. Li, Y., Kurokawa, K., Matsuo, M., Fukuhara, N., Murakami, K. and Sekimizu, K. (2004) Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replication. Mol. Genet. Genomics, 271, 447-457. Detail.
  58. Hamamoto, H., Kurokawa, K., Kaito, C., Kamura, K., Manitra Razanajatovo, I., Kusuhara, H., Santa, T. and Sekimizu, K. (2004) Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. Antimicrob. Agents Chemother, 48, 774-779. Detail.
  59. Oku, Y., Kurokawa, K., Ichihashi, N. and Sekimizu, K. (2004) Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol. Microbiology, 150, 45-51. Detail.
  60. Takeuchi, K., Takahashi, H., Sugai, M., Iwai, H., Kohno, T., Sekimizu, K., Natori, S., Shimada, I. (2004) Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR. J. Biol. Chem., 279, 4981-4987. Detail.
  61. Ichihashi, N., Kurokawa, K., Matsuo, M., Kaito, C. and Sekimizu, K. (2003) Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotidebound to DnaA protein, the initiator of chromosomal DNA replication. J. Biol. Chem., 278, 28778-28786.Detail.
  62. Ubukata, T., Shimizu, T., Adachi, N., Sekimizu, K. and Nakanishi, T. (2003) Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II. J. Biol. Chem., 278, 8580-8585. Detail.
  63. Koyama, H., Ito, T., Nakanishi, T., Kawamura, N. and Sekimizu, K. (2003) Transcription elongation factor S-II maintains transcriptional fidelity and confers oxidative stress resistance. Genes to Cells, 8, 779-788. Detail.
  64. Akimitsu, N., Adachi, N., Hirai, H., Hossain, M. S., Hamamoto, H., Kobayashi, M., Aratani, Y., Koyama, H. and Sekimizu, K. (2003) Enforced cytokinesis without complete nuclear division in embryonic cells depleting the activity of DNA topoisomerase IIalpha, Genes to Cells, 8, 393-402. Detail.
  65. Matsuo, M., Kurokawa, K., Nishida, S., Li, Y., Takimura, H., Kaito, C., Fukuhara, N., Maki, H., Miura, K., Murakami, K. and Sekimizu, K. (2003) Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus. FEMS Microbiol. Lett., 222, 107-113. Detail.
  66. Nakanishi, T. and Sekimizu, K. (2002) SDT1/SSM1, a multicopy suppressor of S-II null mutant, encodes a novel pyrimidine 5'-nucleotidase. J. Biol. Chem., 277, 22103-22106. Detail.
  67. Kaito, C., Akimitsu, N., Watanabe, H. and Sekimizu, K. (2002) Silkworm larvae as an animal model of bacterial infection pathogenic to humans. Microb. Pathog., 32, 183-190. Detail.
  68. Kaito, C. Kurokawa, K., Hossain, M. S., Akimitsu, N. and Sekimizu, K. (2002) Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene. FEMS Microbiol. Lett., 210, 157-164. Detail.